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| Product Name | IMMQUA SCCA2 |
|---|---|
| Generic Name | SCCA2 kit |
| Approval Number | 30100EZX00038000 |
| Product Code | 326076529 |
| Tests per Kit | 48 tests |
| Storage Conditions | 2～8℃ |
| Shelf Life | 1 year |
This kit utilizes an enzyme-linked immunosorbent assay (ELISA) method to quantify SCCA2 in serum. Samples are added to microtiter plate with 96-wells (solid phase) coated with anti-SCCA antibody, and after washing, a POD-conjugated anti-SCCA2 antibody is added to react, an immune complex is formed. After the unreacted POD-conjugated anti-SCCA2 antibody is removed by washing, a chromogenic substrate is added to perform the enzymatic reaction, and color develops in proportion to the amount of SCCA2 in the samples. The color development is stopped, and the color intensity is measured at 450nm.
SCCA2 concentration in the sample bound to the antibody in the solid phase is reflected in the chromogenic amount associated with the reaction between the POD and the chromogenic substrate, and therefore, SCCA2 concentration is determined by measuring this.
(100 µL/well each).
20-30℃ for 16-24 h
[primary reaction]
20-30℃ for 90 minutes
[secondary reaction]
20-30℃, 30 minutes
[chromogenic reaction]
| ① Antibody-coated Plate | 1 pcs |
|---|---|
| ② Standard | 1 mL × 1 |
| ③ Specimen Diluent | 100 mL×1 |
| ④ POD-conjugate | (12 mL)×1 |
| ⑤ Conjugate Solvent | 12 mL×1 |
| ⑥ Color Reagent A | 6 mL×1 |
| ⑦ Color Reagent B | 6 mL×1 |
| ⑧ Stop Solution | 12 mL×1 |
| ⑨ Wash Concentrate | 100 mL×2 |
| ⑩ Control Sample | 1 mL×1 |
| Accessories: Plate seal | 2 pcs |
| Sensitivity | The absorbance of the standard solution is greater at higher SCCA2 concentrations, and the absorbance difference between the 0 ng/mL standard solution and the 0.2 ng/mL standard solution is 1.20 or higher. |
|---|---|
| Accuracy | When a control sample with a known concentration is measured, accuracy is within ±20% of the known SCCA2 concentration is 1.0 ng/mL or higher, and within ±30% of the known SCCA2 concentration is less than 1.0 ng/mL. |
| Simultaneous Reproducibility | When the same sample is measured three times simultaneously, the C.V. value of the measured value is less than 15%. |
| Assay range | 0.3～20 ng/mL |
| Linearity | <±20% |
| Minimum detection sensitivity | 0.3 ng/mL(serum concentration） |
| Cross-reactivity | Does not react with SCCA1 |
| Standard | Human recombinant SCCA2 |
| Sample to be assayed | Human serum |
SCCA(squamous cell carcinoma antigen) is a serine protease inhibitor belonging to serpin superfamily, which is a protein with a molecular weight of about 45 kDa produced mainly by epithelial cells.
SCCA contains the isoforms SCCA1(SERPIN B3) and SCCA2(SERPIN B4, which are independent gene products transcribed from distinct loci, albeit with very high-amino-acid homologies of 91%.It is known that interleukin 13 (IL-13), one of the type Th2 cytokines, plays crucial roles in allergic inflammation. SCCA2 was identified as one of the molecules whose expression is elevated when airway epithelial cells are stimulated with IL-4 and IL-13, cytokines that are central in allergic responses. Serum SCCA2 levels in children with atopic dermatitis have been reported to be significantly elevated according to the severity of the disease, even when compared with existing blood tests. In the treatment of atopic dermatitis, it is important to select and use anti-inflammatory drugs appropriately according to their severity. It was suggested that serum SCCA2 could be a useful marker as an indicator of the severity of childhood atopic dermatitis. Clinical performance test results of product in 159 allergic unaffected patients and 176 patients with atopic dermatitis aged from 0 to 15 years are shown below.