Periostin ELISA Kit

For research use only

Product Name	Periostin ELISA Kit (Human)
Product Code	326070442
Tests per Kit	96 tests×1
Storage Conditions	2～8℃
Shelf Life	2 year

Intended Use

This kit is intended for quantitative determination of human periostin in serum/plasma, cell culture supernatants, nasal lavage fluid, vitreous fluid, tears, sputum, and bronchoalveolar lavage fluid (BALF).

It is configured for research use only, and is not intended for either diagnostic or therapeutic use.
Please read the instructions carefully before use.

Kit Components

(1) Antibody-coated Plate Anti-periostin monoclonal antibody	8 Well × 12 Strips
(2) POD Conjugate (lyophilized) Peroxidase-linked anti-periostin monoclonal antibody	For 12 mL × 1 Vial
(3) Standard (lyophilized) Recombinant human periostin	For 1.0 mL × 1 Vial
(4) Positive Control （lyophilized） Human serum periostin	For 1.0 mL × 1 Vial
(5) Sample Diluent	100 mL × 1 Vial
(6) Conjugate Solvent	12 mL × 1 Vial
(7) Color Reagent A 3, 3’, 5, 5’-tetramethyl-benzidine	6 mL × 1 Vial
(8) Color Reagent B hydrogen peroxide	6 mL × 1 Vial
(9) Stop Solution	12 mL × 1 Vial
(10) 5X Wash Concentrate	100 mL × 2 Vials
(11) Plate Seal	2 Sheets

Kit Features

High Sensitivity: Detection is possible at the picogram level after dilution.
(Limit of quantification: 20 pg/mL)
Low Background: The blank absorbance is below 0.05 Abs.
Specificity: Does not react with βig-h3, or periostin from mouse, bovine, or canine.

Measurement Principles

The assay employs a 2-step sandwich enzyme-linked immunosorbent assay (ELISA). The wells of the microtiter strips were pre-coated with a monoclonal antibody specific for periostin. Samples are added to the wells. Periostin in samples binds specifically to the immobilized antibody. Addition of POD-linked anti-periostin monoclonal antibody forms an antigen-antibody complex to which substrate solution is further added, into the wells, and color develops in proportion to the amount of periostin in the sample. The color development is stopped and the color intensity is then measured at 450 nm.

Outline of measurement procedure

Prepare diluted standards, positive control and diluted samples.

Add 100 µL samples to each well

Incubate for 18–24 hours at 25 ℃

Wash wells 5 times.

Add 100 µL POD-conjugate solution

Incubate for 90 minutes at 25 ℃

Wash wells 5 times.

Add 100 µL substrate solution

Incubate for 10 minutes at room temperature

Add 100 µL Stop Solution

Read the absorbance at 450 nm (primary wavelength)
　　　　　　　　　　　　　　550-700 nm (secondary wavelength)

Measurement specifications

Purpose of Use	Quantification of periostin in human samples (Research Reagent)
Measurement Target	Human periostin
Measurement Method	Sandwich ELISA
Solid-phase Antibody	Monoclonal antibody
Labeled Antibody	Peroxidase-linked monoclonal antibody
Target Specimens	Serum, Plasma (EDTA, citrate, heparin), Bronchoalveolar lavage fluid, Nasal lavage fluid, Tears, Vitreous fluid, Sputum, Cell culture supernatant
Calibration Range	0 to 2 ng/mL
Limit of Quantification	20 pg/mL
Primary Reaction	25℃ for 18-24 hours
Secondary Reaction	25℃ for 90 minutes
Specificity	No cross-reaction with βig-h3 No cross-reaction with mouse, bovine, canine periostin